Anti lamin a antibodies are readily available from several suppliers.
Anti lamin a antibody.
In humans this protein is encoded by the gene lmna.
Additionally anti lamin a c terminal antibodies are suitable for use in immunoblotting approx.
The anti lamin a c antibody reveals strong nuclear lamina staining while anti lamp1 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes.
The full protein is reported to be 664 amino acid residues in length.
Lamins a and c are alternatively spliced versions of the lmna gene.
The protein may also be known as lamin c dhe lamin a cdcd1 cddc and cmd1a.
These proteins are found in many different cell types in three different forms a b and c.
Lamin a c is cleaved by caspase 6 and serves as a marker for caspase 6 activation.
The cleavage of lamins results in nuclear dysregulation and cell death 5 6.
Lamin a c antibodies.
Why is the actual band size different from the predicted.
See machiels et al 1997.
2000 j struct biol 129 313 23.
Lamin a antibody c 3 is a mouse monoclonal igg 2b kappa light chain provided at 200 µg ml.
Anti lamin a c terminal antibody is suitable for use in western blot 0 1 0 2 μg ml using hela nuclear extract.
Anti lamin a c antibodies are available from several suppliers.
As a result this antibody recognizes lamin a but not lamin c.
This antibody can also be used in indirect immunofluorescence 1 2 μg ml using hela cells rat nrk and mouse 3t3 cells.
74 kda observed band size.
It is listed to be a member of the intermediate filament family.
The expected protein mass is 74 1 kda but there are 6 reported isoforms.
The cells were labeled with lamin a monoclonal antibody 133a2 product ma1 06101 at 5 µg ml in 0 1 bsa and incubated overnight at 4 degree and then labeled with goat anti mouse igg h l superclonal secondary antibody alexa fluor 488 conjugate product a28175 at a dilution of 1 2000 for 45 minutes at room temperature panel a.
Anti lamin a antibody ab26300 at 1 1000 dilution hela whole cell extract at 100 µg secondary goat anti rabbit igg h l hrp conjugate at 1 10000 dilution developed using the ecl technique.
Reacts against lamin a exclusively the antibody was raised against the carboxy terminus of 98 amino acids present in lamin a and absent from lamin c.
During apoptosis lamin a c is specifically cleaved into a large 41 50 kda and a small 28 kda fragment 3 4.
Since both dna blue and lamin a c red are associated with the nuclear compartment this region appears crimson in this image.
Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope.
